Vascular Functional Imaging and Physiological Environment of Hyperplasia, Non-Metastatic and Metastatic Breast Cancer
Annual rept. 1 Oct 97-30 Sep 98
JOHNS HOPKINS UNIV BALTIMORE MD
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Our research proposal consists of the following three closely related aims directed towards understanding the role of vascular, physiological and metabolic properties in the metastatic dissemination of breast cancer. Aim 1 To investigate the relationship between metastatic phenotype and vascularization. Aim 2 To investigate the effect of increasing vascularization and permeability on metastasis. Aim 3 To determine the relationship between metastatic phenotype and intra-and extracellular pH and lactate production. In year 2 we have implemented significant technical developments which allow us to interactively display and correlate 3-dimensional MRI information of vascular volume and permeability with 3D reconstructed his to- -logical maps. These developments, instead of the single slice data acquisition we originally proposed, have allowed us to obtain comprehensive information of tumor vascular volume and permeability as well as histological morphology and the relationship between the tow. We have also characterized the metabolism of human mammary epithelial cells HMEC and demonstrated for the first time that HMEC show significantly altered lactate levels and phosphlipid metabolism with malignancy.
- Anatomy and Physiology
- Medicine and Medical Research