Complement Activation By Liposome-Encapsulated Hemoglobin in Vitro: The Role of Endotoxin Contaminative.
WALTER REED ARMY INST OF RESEARCH WASHINGTON DC DIV OF BIOCHEMISTRY
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Incubation of liposome-encapsulated Hb LEH with rat serum at 37C led to accelcelerated decay of serum complement C hemolytic activity CH50. Empty liposomes L caused less expressed decrease of CH50, whereas free Hb had no effect on C hemolytic activity. The LEH- and L-induced increase in C consumption was unlikely due to endotoxin LPS contamination, as spiking of rat serum with LPS caused reduction in CH50 only at levels significantly higher than detectable in LEH or L. The LPS- induced C consumption was not potentiated by free hemoglobin.
- Medicine and Medical Research