The Effect of DNA Methylation on IGF2 Expression.
Final rept. 1 Sep 94-31 Aug 98
GEORGETOWN UNIV WASHINGTON DC
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Our hypothesis is that DNA methyl transferase expression is inversely correlated with IGF-II expression in normal and tumor tissues. That is, tumors with high DNA methyl transferase expression will imprint IGF-II, resulting in reduced IGF-II expression, while tumors with low DNA methyl transferase will have higher relative IGF-II expression. We have demonstrated that DNA methyl transferase expression can be measured readily using RNase protection assay in representative breast cancer cell lines. Sahana Kaup is continuing her graduate studies based on her findings along the outline discussed above. At the present time, her preliminary data have not confirmed her fundamental hypothesis, but further experiments which are currently underway will determine whether or not her original hypothesis remains worthy of investigation.
- Medicine and Medical Research