Accession Number:

ADA361176

Title:

Poliovirus Tumor Vaccine for Breast Cancer Micro-Metastases.

Descriptive Note:

Final rept.,

Corporate Author:

ALABAMA UNIV IN BIRMINGHAM

Personal Author(s):

Report Date:

1998-09-01

Pagination or Media Count:

12.0

Abstract:

The underlying reason for failure to cure patients diagnosed with breast cancer is the presence of micrometastases. The stimulation of anti-tumor immune responses represents one of the more effective ways to treat low tumor burdens that are clinically occult. The objective of our proposal is to determine whether a poliovirus replicon vaccine strategy induces systemic immunity an eradication of micrometastases. During our project, we have constructed and characterized poliovirus replicons which express CE or HER2neu. Mice given these replicons generate anti-CEA or HER2neu antibodies. We had demonstrated protection from tumor challenge in the mice which have antibodies to CEA. The significance of these studies is that we have now characterized the appropriate replicons that will be used to establish route of administration for generating an immune response against CEA HER2neu prior to tumor challenge. We developed companion replicons which express biological active immune modulators GMCSF and IL-2 to increase the immunogenicity of the replicon encoding CEA or HER2neu. Characterization of the immune response to antigens in mice revealed typical TH1 response. The results of our studies provide the foundation for future strategies to us replicons as anti-tumor vaccines.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE