Trichloroethylene Radicals: An EPR/SPIN Trapping Study
Interim rept. Dec 93-Jul 95
ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD
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Trichloroethylene TCE is an environmental contaminant found in the soil and groundwater on several active bases as well as bases scheduled for closure. TCE is a halocarbon and is believed to cause environmental and biological damage through production of free radicals. As part of the process to develop environmental and health effects criteria for base clean-up the initial radicals produced by TCE were studied by electron paramagnetic resonance spectroscopy EPR. Radicals of TCE were formed by gamma-radiation in a 60Co gamma-ray source. TCE at 77 deg K was irradiated at a dose rate of 1 Gymin receiving a total dose of 10 By. The TCE radicals were detected with and without the spin traps 5,5-dimethyl-1-pyrroline-1-oxide DMPO, N-tert-butyl-alpha-phenyl nitrone PBN and 3,5-dibromo-4-nitrosobenzenesulphonate DBNBS. The structure of the spin trapped TCE radical was Cl2CCH. The spin trap of choice for the study of radicals by EPR in biological tissue is PBN. The efficiency of PEN to trap the TCE radical was 20.6.
- Environmental Health and Safety