Breast Tumor Detection Utilizing Novel in situ Amplication Systems.
Annual rept. 7 Jul 97-6 Jul 98
BOSTON UNIV MA
Pagination or Media Count:
The long term goal is to construct in situ, at the site of a tumor. The aggregate will be formed by the sequential administration of small molecules, then a radioactive label will also be delivered by a small molecule. Thus, the tumor to normal tissue radioactivity ratios should be exceptionally high for both diagnostic and therapeutic purposes. The amplification system will consist of two or more classes of multivalent molecules, A, B, C that cannot self assemble but can associate very specifically with each other. This work will take advantage of the known high specificities of single-stranded DNA to bind its complement to form double-stranded structures, of streptavidin to bind biotin, and of pairs of leucine zipppers to dimerize. This project will focus on optimizing the method in model systems and on tumor cells in culture.
- Medicine and Medical Research