Microsatellite Alterations as Molecular Markers in Breast Cancer Progression.
Final rept. 1 Aug 96-31 Jul 98
JOHNS HOPKINS UNIV BALTIMORE MD
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Breast cancer detection and diagnosis has been limited by the lack of early and accurate markers of disease. Efforts to date have largely relied on insensitive measures such as mammography or breast examination for detection, with conventional histopathology for diagnosis of breast cancer. The goal of this project is to utilize new molecular diagnostic techniques to improve breast cancer diagnosis. Towards this goal we evaluated the frequency of genetic alterations in variable short tandem repeats microsatellites in microdissected breast tissue including normal, in situ carcinoma, invasive, and metastatic carcinoma from the same breast lesion. On the same tissues, in parallel, we are testing the utility of a novel marker, the enzyme telomerase. In this way the prognostic and genetic changes that occur within a single breast lesion can be determined. The long range goal of this project is to develop molecular diagnostic techniques which may be used in the detection of breast cancer and to utilize these techniques in defining the genetic alterations associated with neoplastic progression in breast.
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