Effect of Estrogen on Progression of Human Proliferative Breast Cancer Disease in a Xenograft Model
Annual rept. 1 Aug 97-31 Jul 98
MICHIGAN CANCER FOUNDATION DETROIT
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Utilizing the T24-Ha-ras transfected MCF10A xenograft model of early human breast cancer progression we have 1 demonstrated the direct effects of estrogen on growth and sequence of progression of human preneoplastic breast disease, 2 demonstrated the utility and validity of bcl-2, cyclin D1, and c-erbB2 as specific markers for breast cancer progression, and 3 established an in vitro assay system that recapitulates in vivo growth and organization of MCF10AT cells into highly proliferative estrogen responsive ductular elements. Experiments analyzing chemotherapeutic and chemopreventive effects of tamoxifen on growth and progression of MCF10AT lesions are underway. Our data thus far show that transcriptional activation of estrogen receptor gene is not a result of alteration in methylation status of estrogen receptor gene or a position effect of insertion of exogenous T24 Ha-ras gene.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research