Children's Hospital of Pittsburgh Histocotripatibility Center Molecular Typing of Alleles at HLA Class I and Class II Loci on Blood Spotted Filter Paper.
Final rept. 1 Oct 97-30 Sep 98
CHILDREN'S HOSPITAL OF PITTSBURGH PA
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The activities of the Childrens Hospital of Pittsburgh Histocompatibility Center can be classified under three major categories 1 the repository service 2 the molecular typing service and 3 the research activities aimed at improving the technical support on which the previous two services are working. This year in the Repository, where blood samples from volunteers as bone marrow donors are collected from more than 120 recruiting centers nationwide, roughly 150,000 samples were processed. Close to 450,000 vials were stored in three sets of freezers one sample per donor each freezer, while nearly 130,000 were sent out to other Typing Laboratories. In our laboratories, approximately 10,000 typings were performed with a very high Q.C. standard, as monitored by National Marrow Donor Program. Our Centers research branch synthesized all the oligonucleotides to be used as PCR primers or labelled probes to perform molecular HLA typing. DNA sequencing was also performed when hybridization results were not optimal. Alternatives for storing blood samples e.g., blood spots on filter paper or for enhancing typing capabilities e.g., microchip technology to expedite PCR procedures were successfully implemented or improved to the point of justifying their future implementation.
- Medicine and Medical Research