Identification of BRCA1 and 2 Other Tumor Suppressor Genes on Chromosome 17 Through Positional Cloning.
UTAH UNIV SALT LAKE CITY
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The overall goal of our project is to identify genes involved with the development and progression of breast cancer. This goal has remained unchanged since the start of the project, but the discovery of BRCAl in 1994 1 together with technological advances in gene expression profiling 2 has influenced our strategy to achieve this goal. In the early part of the project our search for tumor suppressor genes was directed by genetic or LOH mapping strategies followed by positional cloning of candidate genes. This general approach is still extensively used in many laboratories, but is very labor intensive by nature. Apart from its primary objective of identifying a single cancer gene, this strategy contributes no additional observations relevant to carcinogenesis during the search process itself. A different and much broader strategy for identifying any kind of gene involved in tumor formation and progression, which is not dependent on prior knowledge of location, employs genetic profiling using high density microarrays. Not only does this technique permit detection of the expression profiles of each of a large number of genes in parallel, but it potentially also provides a mechanistic view of how regulatory pathways are controlled.
- Medicine and Medical Research