The Role of Prostaglandin G/H Synthase 2 in the Apoptosis of Human Mammary Epithelial Cells.
Annual rept. 1 Jun 97-31 May 98,
NORTH CAROLINA UNIV AT CHAPEL HILL
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We are examining apoptotic pathways that may be altered in human mammary epithelial cells as a consequence of the overexpression of prostaglandin GH synthase 2. This project involves quantifying the apoptotic response as well as apoptotic regulators ATM, MDM2, p53, bcl-2, and bax in this cell system following exposure to agents that can induce apoptosis. We have shown that human mammary epithelial cells express high levels of prostaglandin GH synthase, are resistant to ionizing radiation-induced apoptosis, but do apoptose when exposed to the broad spectrum kinase inhibitor staurosporine. This report documents progress made in the past year in measuring the apoptotic response in human mammary epithelial cells, in measuring the protein levels of some apoptotic regulators, and in the creation of human mammary epithelial cells that have attenuated levels of prostaglandin GH synthase.
- Anatomy and Physiology
- Medicine and Medical Research