Dual-Specificity Anti-HER-2/neu Antisense DNA Agents for Breast Cancer Therapy.
Annual rept. 1 Jul 97-30 Jun 98
UNIVERSITY OF MEDICINE AND DENTISTRY OFNEW JERSEY PISCATAWAY
Pagination or Media Count:
The purpose of the project is to develop a new class of antisense drug and a procedure for selecting an optimal antisense compound with HER-2neu mRNA as the target. The scope for this year includes preparation of HER-2neu target RNA Task 1, development of a novel peptide synthesis technology for generation of combinatorial libraries Task 2 and preliminary experiments on identifying members of a library that bind HER-2neu RNA Task 3. Task 1 has been somewhat modified in that one representative RNA fragment has been prepared, but this fragment has been further analyzed and found to have sensitive RNase H cleavage sites, which is a highly favorable result. Task 2 has been successfully completed in that a protocol for peptide synthesis in solution phase on disulfide-linked polyethylene glycol has been developed. Task 3 months 9-12 has not yet been initiated due to unanticipated personal problems.
- Medicine and Medical Research