Role of HMG-I (I) in Human Breast Cancer Metastasis
Annual rept. 24 Jun 97-23 Jun 98
WASHINGTON STATE UNIV PULLMAN
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HMG-IY proteins are mammalian architectural transcription factors that regulate transcription of a number of genes. The original proposal is to test a hypothesis that expression of HMG-IY proteins will fundamentally influence the expression of genes, which are involved in metastatic invasion, migration, angiogenesis and colonization. In the second year of study, a great effort has been made to isolate HMG-IY high-expressing breast cancer cell transfectants. These HMG-IY transfectants and HMG-IY monitoring systems are essential tools for further study of metastatic potential and expression of down-stream genes in HMG-IY transfectants. Two new approaches have been developed. a HA-tagged HMG-IY gene constructs were generated. Isolation of stable HMG-IY-expressing clones, including dominant negative HMG-IY clones, by using monoclonal antibody against HA-tag is in progress. b HA-tagged HMG-IY genes were subcloned into a tetracycline inducible gene expression system. The preliminary results indicated that the exogenous HMG-IY proteins in MCF-7 cells could be increased to 50-fold after tetracycline induction.
- Medicine and Medical Research