Transgenic Mouse Model to Study the Role of EGF Receptor in Breast Cancer.
Annual rept. 19 Aug 97-18 Aug 98
ILLINOIS UNIV AT CHICAGO
Pagination or Media Count:
Epidermal growth factor EGF receptor is expressed in many normal tissues, including mammary epithelium. Overexpression or mutation of the EGF receptor causes neoplastic transformation in many cell types. Several studies have implicated the EGF receptor as a good prognostic marker in breast cancer, but its role in the etiology and progression of this malignancy is still under dispute. We propose to utilize a genetic approach to investigate this issue, by developing transgenic mice in which the EGF receptor gene in the mammary gland will be inactivated at the onset of first lactation. The construct for gene targeting was made. After introducing the construct in ES cells by electroporation and G4 18 selection, we have several positive clones for ES cell injection and chimeric mice from some of the positive clones. We hope to generate the transgenic mouse model next year so that we will be able to study the question whether the EGF receptor plays a functional role in the etiology and progression of mammary carcinoma. The answer to this question would indicate the feasibility of using the EGF receptor as a target in breast cancer therapy.
- Anatomy and Physiology
- Medicine and Medical Research