99MTC Alpha-Fetoprotein: A Novel, Specific Agent for the Detection of Human Breast Cancer.
Annual rept. 15 Jun 97-14 Jun 98
ALBANY MEDICAL CENTER HOSPITAL NY
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There is a pressing need for a widely available, non-invasive, and accurate imaging technique to further evaluate mammographically suspicious lesions to reduce the number of cancer negative biopsies. We have demonstrated that technetium-99m radiolabeled human alpha-fetoprotein 99mTc AFP localizes in human breast cancer cells in-vivo, most likely concentrating in breast cancer cells due to a specific receptor not found in normal adult breast tissue. Our technical objective is to assess 99mTc AFP as an agent to detect and stage human breast cancer. As promised in the first two aims of the work scope, we have compared natural full-length and recombinant domain III preparations of 99mTc AFP to clinically used tracers, 201Tl and 99mTc sestamibi, in immune-deficient SCID mice bearing human cancer xenografts. The results of these studies confirm our hypothesis that 99mTc AFP is a superior human breast cancer imaging agent. We have also demonstrated specific uptake of 99rnTc AFP, in-vitro, and have measured the level of AFP receptors in the imaged tumors. During the second year of effort we will continue the work scope for these aims and also determine the ability of 99rnTc AFP to image positive lymph nodes draining a transplantable rat mammary cancer.
- Medicine and Medical Research