Correlative Study of Tumor Hypoxia and Metastatic Potential in Breast Cancer.
Annual rept. 1 Sep 97-31 Aug 98
NORTH CAROLINA UNIV AT CHAPEL HILL
Pagination or Media Count:
We propose a novel physiological approach using tumor hypoxia assessment to study the metastatic potential of primary breast cancer cells. Does the presence of hypoxia in breast primary detected by pimonidazole immunohistochemical binding, correlate with the presence of axillary lymph node metastases, and correlate the presence of hypoxia with markers of cell proliferation, p53, apoptosis, and VEGF in the primary breast tumor tissue. The specific aims are 1 Determine the presence and extent of tumor hypoxia in biopsies of primary breast cancer using pimonidazole binding to hypoxic tumor cells. 2 Determine the patterns of pimonidazole binding in the breast cancer biopsies in relation to other landmarks such as blood vessels and necrosis. 3 Correlate the presence and extent of tumor hypoxia in primary breast cancer with the presence of axillary node metastases. 4 Correlate the presence and extent of tumor hypoxia with the presence of other biological markers p53, apoptosis, PCNA, Ki-67, and VEGF. 5 Monitor adverse effects of pimonidazole. The clinical protocol has not been initiated. Please see the Annual Report Statement. We request deferral of the start date of the clinical research protocol to November 1st, 1998 or earlier pending Review Committee approval.
- Anatomy and Physiology
- Medicine and Medical Research