Accession Number:

ADA355444

Title:

Complement Activation by Liposome-Encapsulated Hemoblobin in Vitro: The Role of Endotoxin Contamination.

Descriptive Note:

Corporate Author:

WALTER REED ARMY INST OF RESEARCH WASHINGTON DC DIV OF BIOCHEMISTRY

Personal Author(s):

• Szebeni, Janos
• Wassef, Nabila M.
• Rudolph, Alan S.
• Alving, Carl R.

Report Date:

1994-01-01

Pagination or Media Count:

11.0

Abstract:

Incubation of liposome-encapsulated Hb LEH with rat serum at 37 deg C led to accelerated decay of serum complement C hemolytic activity CH50. Empty liposomes L caused less expressed decrease of CH50, whereas free Hb had no effect on C hemolytic activity. The LEH- and L-induced increase in C consumption was unlikely due to endotoxin LPS contamination, as spiking of rat serum with LPS caused reduction in CH50 only at levels significantly higher than detectable in LEH or L. The LPS-induced C consumption was not potentiated by free hemoglobin.

Descriptors:

• *LIPOSOMES
• *IN VITRO ANALYSIS
• *CONTAMINATION
• *HEMOGLOBIN
• *ENDOTOXINS
• RATS
• CONSUMPTION
• BLOOD SERUM
• DECAY
• ACCELERATED TESTING
• HEMOLYSIS

Subject Categories:

• Biochemistry
• Anatomy and Physiology
• Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE