Accession Number:

ADA355334

Title:

Role of a Placenta-Specific Gene in Mammary Tumorigenesis.

Descriptive Note:

Annual rept. 1 Jul 97-1 Jul 98

Corporate Author:

HEALTH RESEARCH INC BUFFALO NY

Personal Author(s):

Report Date:

1998-08-01

Pagination or Media Count:

18.0

Abstract:

We have previously shown that mouse mammary preneoplasias and carcinomas of several etiologies ectopically express 2.2 and 5.6 kb MIPP-related mRNAs. This raises the possibility that MIPP might be a mouse mammary oncogene. Furthermore, MIPP is assigned to the actin-binding kelch family based on sequence analysis. However, the products of the MIPP gene has not yet been identified. Therefore, the purpose of this research is to clone the 2.2 and 5.6 kb RNAs for use in transformation assays and functional studies. The 5 end of the 2.2 kb transcript was cloned by RACE using the SMART oligonucleotide Clontech. The cDNA contained a 1.7 kb open reading frame coding for 556 amino acids. Sequence analysis revealed the presence of an N-terminal BTB proteinprotein interaction domain plus six kelch repeats at the C-terminus. An epitope-tagged recombinant MIPP protein is being expressed in EL12 mouse mammary epithelial cells to determine 1 whether they become transformed, and 2 its subcellular location and interaction with actin. Efforts are also focused on cloning the 5.6 kb mammary carcinoma-specific transcript. This work could lead to a better picture of how derangement of the actin cytoskeleton relates to mammary tumorigenesis.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE