Accession Number:

ADA355181

Title:

Three-Dimensional Structure Determination of Botulinum Toxin.

Descriptive Note:

Annual rept. 1 Aug 97-30 Jun 98

Corporate Author:

CALIFORNIA UNIV RIVERSIDE

Personal Author(s):

• Stevens, Ray C.

Report Date:

1998-07-01

Pagination or Media Count:

60.0

Abstract:

The immediate goals of the contract on the structure and function relationship of botulinum neurotoxin are 1 Determine the three-dimensional structure of botulinum neurotoxin at atomic resolution by x-ray crystallography. 2 Based on the structure of the neurotoxin, understand the toxins mechanism of action. We have accomplished the first goal of determining the three-dimensional structure of the 150 kD botulinum neurotoxin serotype A. The toxin is Y-shaped, with a very long alpha-helical translocation domain forming the backbone of the structure. The translocation domain is composed almost entirely of helices, 2 of which are 95 A in length and form a pseudo-coiled coil. The binding domain and catalytic domain are more globular in shape, located at two different ends of the translocation domain. The overall dimensions of the protein are 120 A x 80 A x 40 A. A complete description of the three- dimensional structure is described in the report the manuscript will he published in the September issue of Nature Structural Biology. We have recently collected data on an inhibitor complex and a receptor - complex. Refinement and analysis of the toxin with bound molecules will be completed within the next 12 months.

Descriptors:

• *X RAYS
• *THREE DIMENSIONAL
• *TOXINS AND ANTITOXINS
• *CRYSTALLOGRAPHY
• *CLOSTRIDIUM BOTULINUM
• CONTRACTS
• SIZES(DIMENSIONS)
• BIOLOGY
• MOLECULES
• STRUCTURAL PROPERTIES
• PROTEINS
• RESOLUTION
• VERTEBRATES
• CATALYSIS
• INHIBITORS
• ATOMIC PROPERTIES
• DETERMINATION
• SEROLOGY
• BACTERIAL TOXINS
• CNS DEPRESSANTS
• TRANSLOCATION

Subject Categories:

• Toxicology
• Microbiology
• Crystallography

Distribution Statement:

APPROVED FOR PUBLIC RELEASE