Tissue Banks and Cell Lines Derived from Patients with a High Risk of Breast Cancer and in situ Disease
Final rept. 1 Nov 94-1 May 98
ROYAL CANCER HOSPITAL LONDON (UNITED KINGDOM) INST OF CANCER RESEARCH
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This project is aimed at establishing bulk cultures of individual epithelial and stromal cells from mastectomy specimens of patients who are carrying genes that predispose them to breast cancer. Epithelial and stromal cells are being conditionally immortalized using an amphotropic retroviral vector transducing the tsA58-U19 SV40-derived construct. These cells are from patients who are mutation carriers of BRCA1, BRCA2, and p53. Samples have been received from 46 women, five of which have a Li-Fraumeni - phenotype, with confirmed mutations. Seven cases are BRCA1 carriers, four with confirmed mutations. In the majority of cases primary cultures have been established, cells banked and immortalization commenced. In parallel a detailed data-base has been established of in situ disease from patients that have been sent to the Royal Marsden Hospital Breast Unit since 1970. Over 5,500 slides have been reviewed of in situ breast cancer from over 700 cases. Material from over 500 patients has been selected for further study. The cases from 1994-96, with short follow up are being used to pilot the molecular studies. This material is being used for Comparative Genomic Hybridisation CGH and allelotyping, for immunohistochemical studies and in a joint European Union funded project to develop a CD ROM to produce a uniform classification of in situ disease. Discussion are underway with Dr R. Cardiff and Dr L. Henninghausen at the NCI to incorporate these cases onto a WEB page as an educational package.
- Anatomy and Physiology
- Medicine and Medical Research