Development of New Approaches for Breast Cancer Therapy and Diagnosis Based on Angiogenesis
Annual rept. 30 Sep 96-20 Sep 97
CHILDREN'S HOSPITAL MEDICAL CENTER BOSTON MA
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This project focuses on angiogenesis, the growth of new blood capillaries, as a potential target for breast cancer therapy and diagnosis. Our approach is based on our past demonstration that many of the signal transducing molecules that mediate the effects of angiogenic mitogens and extracellular matrix molecules are concentrated within specialized cell adhesion sites, known as the focal adhesion complexes FACs. Over the past year, we began to develop and screen monoclonal antibodies using isolated FACs as an immunogen. Positive hybridoma lines have been identified that label FAC-associated proteins in adherent GE cells and these clones have been isolated. Production of these antibodies and identification of molecules that mediate growth modulation in capillary cells should facilitate development of new angiogenesis inhibitors as well as alternative methods for breast cancer diagnosis.
- Anatomy and Physiology
- Medicine and Medical Research