Accession Number:

ADA346801

Title:

Non-Invasive Detection of Axillary Nodes by Contrast Enhanced Magnetic Resonance Imaging.

Descriptive Note:

Annual rept. 16 Sep 96-15 Sep 97

Corporate Author:

SLOAN-KETTERING INST FOR CANCER RESEARCH NEW YORK

Personal Author(s):

Report Date:

1997-10-01

Pagination or Media Count:

15.0

Abstract:

Previous studies in murine tumors have shown that the 3 drug combination of PALA MMPR 6AN PALA n-phosphonacetyl aspartate, MMPR 6-methylmercaptopurine riboside, 6AN 6-aminonicotinamide referred to as PMA is an effective radiation sensitizer. Using the MCF-7 human tumor, we have studied the effects of these drugs on metabolism and cell survival. In vivo studies demonstrate the presence of 6-phosphogluconate 6PG and a reduction in nucleoside triphosphates after treatment with PMA. MCF-7 tumors were found to be more sensitive to radiation than previously studied murine tumors. PMA is more toxic to nude mice than to C3H mice but appears to be tolerable, although further studies are ongoing. In vitro studies with MCF-7 cells demonstrated that 6AN is not toxic by itself, but does induce metabolic inhibition, based on depletion of NTP and the appearance of 6PG. Ongoing in vivo studies include completion of the 31P NMR metabolic studies and tumor growth delay assays. In vitro studies include cell survival studies on the effect of 6AN in enhancing radiation response.

Subject Categories:

  • Medicine and Medical Research
  • Pharmacology
  • Acoustics

Distribution Statement:

APPROVED FOR PUBLIC RELEASE