Characterization of Breast Cancer Progression by Analysis of Genetic Markers.
Annual rept. 30 Sep 96-29 Oct 97,
ARMED FORCES INST OF PATHOLOGY WASHINGTON DC
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Genetic changes implicated in the etiology of breast cancer have been identified by the detection of loss of heterozygosity at specific loci. Our study utilizes a series of genetic polymorphisms detectable by the polymerase chain reaction PCR to look for changing patterns of LOH as breast cancer progresses from intraductal to invasive and then to metastatic disease. The method involves the microdissection each tumor component present from a panel of breast cancer cases, and then to test each component for LOH at loci that are known to show high frequency LOH in breast cancer. The aims were to determine where in progression LOH is first observed and to determine whether LOH correlates with the clinical behavior of the tumor. We have now completed the analysis of LOH at loci on 3p, 9p, 11p, 13q, 16q, 17p, and 17q for a panel of 115 breast cancers. At each locus examined, LOH, when present at all, is usually seen in the intraductal component of the tumor and maintained throughout tumor progression. LOH at 11p was found to show no correlations with the clinical behavior of the tumor. Clinical correlations of LOH at the other loci are currently being analyzed.
- Medicine and Medical Research