Accession Number:

ADA346327

Title:

Acoustic Microsensors III. Direct Detection of Staphylococcal Enterotoxin B Employing a Piezoelectric Crystal Immunosensor with a Flexible Carboxylated Dextran Matrix as the Biochemical Interface.

Descriptive Note:

Final rept.,

Corporate Author:

PRINS MAURITS LABORATORIUM TNO RIJSWIJK(NETHERLANDS)

Personal Author(s):

Report Date:

1998-03-01

Pagination or Media Count:

25.0

Abstract:

Within the framework of the project Acoustic Microsensors A93KL448 the possibilities of a biosensor system based on an immunological reaction on a piezoelectric crystal sensor are investigated at the TNO Prins Maurits Laboratory TNO-PML. The results described are a continuation of previous reported work in which the toxin Staphylococcal Enterotoxin B SEB was detected in a competition assay with a preincubation period of at least 20 minutes and a detection limit of 100 ngml. A new biochemical interface based on a flexible carboxylated dextran matrix, to which antibodies are bound, was developed. With this biosensor system SEB could be detected directly without a preincubation step at a lowest concentration of approximately 7 ngml. The biosensor can be easily regenerated by a one-minute injection of 0.1 M HCl.

Subject Categories:

  • Toxicology
  • Microbiology
  • Crystallography

Distribution Statement:

APPROVED FOR PUBLIC RELEASE