Acoustic Microsensors III. Direct Detection of Staphylococcal Enterotoxin B Employing a Piezoelectric Crystal Immunosensor with a Flexible Carboxylated Dextran Matrix as the Biochemical Interface.
PRINS MAURITS LABORATORIUM TNO RIJSWIJK(NETHERLANDS)
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Within the framework of the project Acoustic Microsensors A93KL448 the possibilities of a biosensor system based on an immunological reaction on a piezoelectric crystal sensor are investigated at the TNO Prins Maurits Laboratory TNO-PML. The results described are a continuation of previous reported work in which the toxin Staphylococcal Enterotoxin B SEB was detected in a competition assay with a preincubation period of at least 20 minutes and a detection limit of 100 ngml. A new biochemical interface based on a flexible carboxylated dextran matrix, to which antibodies are bound, was developed. With this biosensor system SEB could be detected directly without a preincubation step at a lowest concentration of approximately 7 ngml. The biosensor can be easily regenerated by a one-minute injection of 0.1 M HCl.