Breast Cancer Cell Metabolism Studied by MRS.
Annual rept. 1 Jul 96-30 Jun 97
GEORGETOWN UNIV WASHINGTON DC
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It is proposed to study the metabolism of intact breast cancer cells using non-invasive magnetic resonance spectroscopy MRS methods. Magnetic resonance imaging MRI has been shown to have some value in assessing breast lumps, but lacks specificity. MRS can in principle provide this specificity. In order to evaluate the potential of MRS, systematic studies will be carried out on cell lines selected for the progressive estrogen independent phenotype and the effects of hormones and tamoxifen. The cell studies will provide basic information on the metabolic profile of different BC cell lines in the progression from estrogen and drug sensitive to estrogen independent and drug resistant phenotypes. Cells are embedded in gels and are examined in the MR spectrometer while undergoing perfusion. Previous studies have mainly used carbohydrate agarose gels. But, based on preliminary results, we will use a protein gel Matrigel for which the cells have surface receptors, so that their metabolism can be monitored while they are proliferating. This allows the effects not only of different phenotypes, but also of hormones, and antiestrogens such as tamoxifen, as well as certain drugs and drug combinations to be evaluated. Both 31P and proton MR methods will be applied. The results will be of significance not only to understanding the fundamental biological processes involved, but also to the observation of in vivo MRS of breast lumps.
- Anatomy and Physiology
- Atomic and Molecular Physics and Spectroscopy
- Nuclear Physics and Elementary Particle Physics