Accession Number:

ADA343415

Title:

Environmental Genome Analysis: Polymerase Chain Reaction Fingerprinting on In Situ Extracted Bacterial mRNA from Contaminated Soils.

Descriptive Note:

Final Technical rept. 1 May 94-31 Jan 97

Corporate Author:

TENNESSEE UNIV KNOXVILLE

Personal Author(s):

Report Date:

1998-03-31

Pagination or Media Count:

72.0

Abstract:

The goal of this research was to develop a polymerase chain reaction method to fingerprint mRNA obtained by in situ extraction from contaminated soils to assess the differential catabolic gene expression of a soil microbial population. This approach, which is now generally referred to in the scientific community as differential display DD, has great potential application for the study of gene expression in the environment and the acquisition of novel genetic elements for industrial application. The major outcomes of this work are 1 Optimization of DD for prokaryotic cells. 2 Demonstration of DD from soil derived RNA obtained from both inoculated and uninoculated soil microcosms. In the process of achieving these goals several previously uncharacterized genetic elements were isolated and cloned.

Subject Categories:

  • Biochemistry
  • Toxicology
  • Microbiology
  • Soil Mechanics

Distribution Statement:

APPROVED FOR PUBLIC RELEASE