Environmental Genome Analysis: Polymerase Chain Reaction Fingerprinting on In Situ Extracted Bacterial mRNA from Contaminated Soils.
Final Technical rept. 1 May 94-31 Jan 97
TENNESSEE UNIV KNOXVILLE
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The goal of this research was to develop a polymerase chain reaction method to fingerprint mRNA obtained by in situ extraction from contaminated soils to assess the differential catabolic gene expression of a soil microbial population. This approach, which is now generally referred to in the scientific community as differential display DD, has great potential application for the study of gene expression in the environment and the acquisition of novel genetic elements for industrial application. The major outcomes of this work are 1 Optimization of DD for prokaryotic cells. 2 Demonstration of DD from soil derived RNA obtained from both inoculated and uninoculated soil microcosms. In the process of achieving these goals several previously uncharacterized genetic elements were isolated and cloned.
- Soil Mechanics