Spatial Distribution of the EGF Receptor in Regulation of Breast Epithelial Cell Growth and Organization.
Annual rept. 1 Sep 96-31 Aug 97,
UTAH UNIV SALT LAKE CITY
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Over the last year, we have make progress in our efforts to understand the role of the EGFR distribution in both tissue organization and cancer. We have caused mislocalization of EGFR in polarized epithelial cells and found that regulation and down regulation of the EGFR are distinct from apical and basolateral surfaces. Furthermore, substrate phosphorylation is distinct from the two cell surfaces. We have also extended our studies on the transmodulation of the erbB-2 gene product by the EGFR. We found that overexpression inhibits down regulation of erbB-2 triggered by EGFR activation. In addition, overexpression of erbB-2 also inhibits normal downregulation of the EGFR, apparently by inhibiting lysosomal targeting. Finally, we have improved our methodologies for expressing mutant forms of EGFR and its ligands in non-transformed HMEC, providing a basis for further studies on the role of FGFR trafficking in HMEC physiology.
- Anatomy and Physiology
- Medicine and Medical Research