Line-1 Retrotransposons as Mutagens in Human Breast Cancer.
Annual rept. 30 Sep 96-29 Sep 97,
ARMED FORCES INST OF PATHOLOGY WASHINGTON DC
Pagination or Media Count:
The human LINE-1 retrotransposon L1Hs is known to cause mutations by inserting into genes and inactivating them. The element is expressed in many breast tumors and breast mor cell lines, suggesting that L1Hs-induced mutations may play some role in this malignancy. To test this hypothesis we plan to place a tagged LlHs element in non- malignant breast epithelial cells and let it transpose. If the cells become malignant, we can isolate the genes into which the element has transposed by using the unique tag. Previously we tested a number of plasmid and retroviral vectors in several cell lines without success. We have recently found that a non-integrating tagged LlHs vector may be useful if we can succeed in increasing the number of retrotransposition eventstransfected cell population. In addition, we are using the expression vector pIRESineo producing a bicistronic niRNA to express L1Hs-encoded proteins in transfected MCFiOA cells. This system will be useful to test the effects of expressionoverexpression of L1Hs-encoded proteins in non-malignant cells.
- Anatomy and Physiology
- Medicine and Medical Research