Electrochemical Treatment of Breast Cancer with Direct Current.
Annual rept. 1 Oct 96-30 Sep 97,
CITY OF HOPE NATIONAL MEDICAL CENTER DUARTE CA
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Electrochemical treatment ECT of cancer is a promising new method which delivers direct current dc into tumor tissue by inserting electrodes anodes andor cathodes to induce tumor regression. The purpose of this study is to evaluate the effectiveness of ECT and tissue responses to anode and cathode. Methods I Tumor tissue responses to ECT 1 x 10 rat breast cancer cells MTF-7 were injected subcutaneously into the right fat pad of the mammary gland of a Fisher 344 female rat. While the tumors were grown to 2 x 2 c 2 cm, 60 rats were divided into 6 groups and underwent ECT with doses of 0, 5, 10, 20, 30, and 40 coulombs C, respectively 9 in each group except 12 in 5 and 10 C groups. Two platinum electrodes were inserted at a 1-cm spacing into the tumor. Three tumor samples in each group were removed at 0, 24, and 18 hrs post-treatment for light microscopy and at 0 hour for electron microscopy. 2 Normal muscle tissue response to ECT 9 Fisher 314 female rats were divided into 3 groups anode, cathode, and both anode and cathode insertion in right thigh muscle 3 in each group with 10 C applied. results There are distinct changes to the cellular structure of the treated tumors. The changes ranged from blurred to completed loss of cellular outlines. An abrupt transition from treated tumor to viable tumor indicated that there is a definite effective treatment area, based upon the close applied. The area adjacent to the cathode exhibited minor swelling, while dehydration ,as observed around the anode. Also, We observed disruption in the treated muscle tissue, either with cathode, anode, or both. Conclusions I ECT is effective for cellular disruption of the acted tissue 2 both anode and cathode can damage and destroy cell structures 3 The effects of ECT are nonspecific.
- Medicine and Medical Research
- Physical Chemistry