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Neural Responses to Injury: Prevention, Protection and Repair; Volume 8: Vision, Laser Eye Injury, and Infectious Diseases.

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Annual rept. 20 Sep 95-19 Sep 96,

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The experimental animals used during this period for the project, Neural Responses to Injury Prevention, Protection, and Repair, Subproject Vision, Laser Eye Injury and Infectious Diseases, are as follows Species Used Cynomolgus monkey, squirrel monkey, Squirrel monkey SPECIFIC AIMS Study a new confocal microscope that can be used in living eyes to understand the earliest stages of trauma, laser injuries and diseases. Evaluate drugs to prevent retinal damage after laser injury Study traumatic and non-traumatic glaucoma to determine its pathogenesis Study drugs that will prevent the recurrences of ocular herpes. Studies AND RESULTS Confocal Microscopy 1. Development of the confocal microscope a. Three-dimensional reconstruction of images using real-time confocal microscopy Confocal microscopy is an imaging technique that allows non-invasive optical sectioning of semi-transparent biological specimens 1-16. It offers improved lateral and axial resolution, as well as superior out-of-focus noise rejection. The cornea, which is a transparent structure contiguous with the sclera at the anterior pole of the eye, is particularly well suited for examination by confocal microscopy. It is approximately 0.52 mm thick and is composed of five layers the corneal epithelium, which is the outside layer that is in contact with the tear film Bowmans layer the stroma Descemets layer and the corneal epithelium. By far the largest component is the stoma, which constitutes about 90 of the thickness of the cornea. Although corneas of experimental animals and humans have been successfully imaged in vivo by confocal microscopy, a full three-dimensional reconstruction of the living human cornea has never before been accomplished.

Subject Categories:

  • Animal Husbandry and Veterinary Medicine
  • Anatomy and Physiology
  • Medicine and Medical Research

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