Accession Number:

ADA339032

Title:

Genetic Determinants of Sindbis Virus Oral Infectivity in Aedes Aegypti Mosquitoes

Descriptive Note:

Doctoral thesis

Corporate Author:

AIR FORCE INST OF TECH WRIGHT-PATTERSONAFB OH

Personal Author(s):

Report Date:

1998-03-09

Pagination or Media Count:

129.0

Abstract:

The TE32J double subgenomic Sindbis viruses dsSIN have been used to express genes in Aedes Aegypti nerve and salivary gland tissues. However, TE32J viruses must be intrathoracically inoculated into the mosquitoes and exogenous expression in the mosquito midgut has not been possible. To improve oral infectivity, serial oral passage with selection for variants that disseminated to neural tissues resulted in an eight-fold increase in dissemination rate and enhanced tropism for mosquito midgut epithelium. Sequencing of the glycoprotein- encoding genes from the passaged virus revealed three consensus amino acid changes.

Subject Categories:

  • Genetic Engineering and Molecular Biology
  • Biology
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE