99: A Novel Myc-Interacting Protein with Features of a Breast Tumor Suppressor Gene Product.
Annual rept. 15 Sep 96-14 Sep 97
PENNSYLVANIA UNIV PHILADELPHIA WISTAR INST
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Bin1 is a novel tumor suppressor-like molecule we identified through its ability to interact with and inhibit the oncogenic activity of the Myc oncoprotein, which is widely deregulated in breast cancer. Last year, we proposed to test the hypothesis that Bin1 was a breast tumor suppressor molecule. In year one of the project, we completed characterization of Bin1 antibodies useful for immunohistochemistry and the cloning and characterization of the entire greater than or equal 53 kb human Bin1 gene and 5 flanking region. Using these reagents, we demonstrated that 1 Bin1 is expressed in normal breast epithelial cells but very frequently missing in malignant breast tumors, and that 2 the Bin1 gene was altered as the potential basis for lack of expression in 316 tumor cell lines examined. In preparation to assess the functional of genetic abnormalities whose identification is ongoing, we developed mechanism-based assays for malignant growth inhibition by Bin1. Finally, in preparation for biological analyses, we developed a recombinant Bin1 adenovirus and an MCF7 inducible gene system.
- *BREAST CANCER
- CLINICAL MEDICINE
- MOLECULAR BIOLOGY
- MEDICAL SERVICES
- GENETIC ENGINEERING
- MAMMARY GLANDS
- ONCOGENIC VIRUSES
- Anatomy and Physiology
- Medicine and Medical Research