Pgp Concentration of Carcinogens in Breast Epithelia.
Annual rept 1 Sep 96-31 Aug 97
MEDICAL UNIV OF SOUTH CAROLINA CHARLESTON
Pagination or Media Count:
We are examining the possibility that the membrane transporter, Pgp, could continually or episodically under the influence of various steroid hormones or environmental factors transport hydrophobic toxic molecules, including pesticides, into the lumen of breast epithelia over long periods of time years. Toxins transported into the lumen would be concentrated well above blood concentrations and would continue to diffuse from the lumen back to the cell, affording them time to damage or modify DNA. Published immunocytochemical studies reveal that Pgp is resent in human breast ductules, but we have not seen any functional evidence for its presence. We have preliminary evidence for another transports, the multispecific organic anion transporter MOAT, which transports fluorescent methotrexate into ductule lumens. A second phase of this research, utilizing primary tissue cultures and cell lines of human breast epithelial tissue. We will use these model systems to determine transporter substrates and potential hormonal regulation of these transporters.
- Anatomy and Physiology
- Medicine and Medical Research