Accession Number:

ADA334885

Title:

Regulation of Growth Factor Release By Proteases Inhibitors in Breast Cancer

Descriptive Note:

Annual rept 15 Aug 96-14 Aug 97

Corporate Author:

NEW YORK UNIV MEDICAL CENTER NY

Personal Author(s):

• Finlay, Thomas H.

Report Date:

1997-09-01

Pagination or Media Count:

18.0

Abstract:

This report describes the cloning, sequencing and properties of a potential TGFa-releasing protease present in human breast epithelial cells. Degenerate oligonucleotides based on the conserved sequences about the his57and ser195 residues in mammalian serine proteases were used to clone a novel serine protease sequence PCR-7 from an MCF-7 breast cancer cell cDNA library. The deduced 321 amino acid open reading frame shows a high degree of homology with the cell-surface serine proteases enterokinase, prekalikrein, hepsin and prostasin. Northern blot analysis, using a PCR-7 cDNA probe, shows the presence of an approximately 4.2 kb mRNA species in total RNA from MCF-7 cells.

Descriptors:

• *CLONES
• *GROWTH(PHYSIOLOGY)
• *BREAST CANCER
• EPITHELIUM
• PROTEINS
• DEOXYRIBONUCLEIC ACIDS
• GENES
• GENETIC ENGINEERING
• CELLS(BIOLOGY)
• RIBONUCLEIC ACIDS
• AMINO ACIDS
• OLIGOMERS
• MAMMARY GLANDS
• PEPTIDE HYDROLASES
• SERINE
• NUCLEOTIDES
• ENZYME INHIBITORS

Subject Categories:

• Biochemistry
• Genetic Engineering and Molecular Biology
• Anatomy and Physiology
• Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE