Regulation of Growth Factor Release By Proteases Inhibitors in Breast Cancer
Annual rept 15 Aug 96-14 Aug 97
NEW YORK UNIV MEDICAL CENTER NY
Pagination or Media Count:
This report describes the cloning, sequencing and properties of a potential TGFa-releasing protease present in human breast epithelial cells. Degenerate oligonucleotides based on the conserved sequences about the his57and ser195 residues in mammalian serine proteases were used to clone a novel serine protease sequence PCR-7 from an MCF-7 breast cancer cell cDNA library. The deduced 321 amino acid open reading frame shows a high degree of homology with the cell-surface serine proteases enterokinase, prekalikrein, hepsin and prostasin. Northern blot analysis, using a PCR-7 cDNA probe, shows the presence of an approximately 4.2 kb mRNA species in total RNA from MCF-7 cells.
- Genetic Engineering and Molecular Biology
- Anatomy and Physiology
- Medicine and Medical Research