Accession Number:

ADA334403

Title:

Molecular Study of Interactions Between P-Glycoprotein and Anticancer Drugs.

Descriptive Note:

Final rept. 1 Aug 94-31 Jul 97

Corporate Author:

TEXAS UNIV AT GALVESTON

Personal Author(s):

Report Date:

1997-08-01

Pagination or Media Count:

20.0

Abstract:

P-glycoprotein is a plasma membrane protein that functions as a drug transporter and is responsible for multidrug resistance in some breast cancers. In the past one year, we have generated two site-specific antibodies and used them to determine the topologies of P-glycoprotein in multidrug resistant cancer cells. We found that P-glycoproteins in the plasma membrane of mammalian cells express at least two alternate topologies. This observation is consistent with our previous study using cell-free expression system. The more than one topology feature of P-glycoprotein may be responsible for its multifunctional nature. We have also been able to express the transmembrane domains of P-glycoprotein in bacteria. The success in this study will allow us to map the drug-binding domain in P-glycoprotein and study the drug-P-glycoprotein interactions.

Subject Categories:

  • Medicine and Medical Research
  • Toxicology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE