Medium for Detecting Toxic Strains of Cl. Perfringens Type A, B, C, D, F - USSR -.
JOINT PUBLICATIONS RESEARCH SERVICE ARLINGTON VA
Pagination or Media Count:
The simplest methods of isolation of Cl. porfringens Wilson-Blair media Robinson-Stoval, high column, etc. have a substantial defect -- they do not permit the separation of the toxigenic and nontoxigenic strains in the primary inoculation 2. Hence, at the present time, in order to isolate Cl. perfringens from various sources one makes extensive use of the methoas based on the utilization of properties of the majority of the toxigenic strains of this microbe to secrete hemolysins and leclthinase 2, 3. The divided inoculation of the investigated material on various indicator media -- dishes with blood-agar, dishes with yolk agar, etc. 3 -- requires a large consumption of media and laboratory dishware. The specificity of this method is not very high, since many clostridia and other microorganisms are capablo of causing hemolysis of erythrocytes and the splitting of lecithin. Divided inoculation of the material does not enable ore to state well any certainty that a colony surrounded by zones of hemolysis is capable of a simultaneous alteration of lecithin.