Suppressor Genes in Breast Cancer
Annual rept 1 Jul 96-30 Jun 97
GEORGETOWN UNIV WASHINGTON DC
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The current proposal will focus on the isolation and characterization of novel TSGs in human breast cancer. A human epithelial eukaryotic cDNA expression library has been constructed, and will be transfected into the human breast cancer cell line MCF-7. Genes inhibiting the growth of MCF-7 cells will be considered candidate TSGs for breast cancer. These will be cloned and the full length cDNA sequence obtained. Expression of cloned genes will first be investigated in RNA populations derived from two immortalized normal human breast epithelial cell lines AlN4 and MCF-lOA, and in MCF-7 cells growth arrested either by antiestrogen- treatment or estrogen withdrawal.
- Genetic Engineering and Molecular Biology
- Anatomy and Physiology
- Medicine and Medical Research