Accession Number:

ADA332016

Title:

Glycosphingolipids as Putative Receptor for Staphylococcal Enterotoxin-B in Cultured Human Kidney Cells.

Descriptive Note:

Final rept. 1 Aug 94-31 Jul 97,

Corporate Author:

JOHNS HOPKINS UNIV BALTIMORE MD SCHOOLOF MEDICINE

Personal Author(s):

Report Date:

1997-08-01

Pagination or Media Count:

25.0

Abstract:

Staphylococcal enterotoxin-B SEB is a common enterotoxin that can cause diarrhea and death in man. In these studies we have developed a specific and sensitive assay for the detection of SEB enzyme linked receptor-based immunodot in human fluids, plasma and urine. Our structurefunction studies have revealed that amino acid sequence 130-160 of SEB peptide 9 imparts toxic effects including cell death in PT cells. In addition, we found that SEB can activate neutral sphingomyelinase N-SMase resulting in the hydrolysis of sphingomyelin to ceramide and phosphocholine. Ceramide, in turn induces programmed cell death apoptosis. Interestingly, several of the SEB mutants of peptide 9 were found to abrogate SEB toxicity in human kidney cells. Our findings will of potential value for the food industry, and to help determine toxemia in our soldiers. Such studies will also elaborate the pathophysiology of SEB induced toxemia in man.

Subject Categories:

  • Personnel Management and Labor Relations
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE