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Effects of Shifts in Cell Surface Properties on Adhesion and Activity in Engineered Bioluminescent Bacteria in Biofilms.

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Final Technical rept. 1 Jul 94-30 Jun 97

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Shifts in the membrane composition of two strains of Pseudoronas putidasolvent sensitive strain MW 200 and solvent tolerant strain Idaho were studied following exposure to solvent o-xylene. Responses to solvent were examined in terms of phospholipid and fatty acid content increasing in strain Idaho, and decreasing in strain MW1200, and synthesis and composition of lipopolysaccharide again increasing in strain Idaho and decreasing in strain MW1200. Possibly due to the alteration in LPS structure, stable biofilms of strain Idaho formed more quickly in the presence of solvent than was observed in the absence of solvent. Also, changes in the biosynthesis rate of the phospholipids in the two strains were measured. Both strains exhibited an increase in biosynthesis rate in the presence of o-xylene with the greatest increase in strain Idaho. The increased rate of biosynthesis is highly beneficial to the Idaho cells enabling rapid repair of membrane damage and, therefore an increased solvent tolerance with up to on 100 fold decrease in permeability to solvent. Although a nah-lux construction was made and stressfully inserted onto the strain Idaho chromosome, the transformant produced light under all conditions. Consequently, it was not usable for attachment response studies following changes in the bulk phase composition.

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  • Biochemistry
  • Microbiology

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