Accession Number:

ADA331130

Title:

Regulation of Anchorage-Independent Growth in Breast Cancer: Role of Signalling by Extracellular Matrix and Growth Factors

Descriptive Note:

Final rept. 1 Aug 94-29 Jul 97

Corporate Author:

QUEEN'S UNIV KINGSTON (ONTARIO)

Personal Author(s):

• Saulnier, Ron

Report Date:

1997-07-01

Pagination or Media Count:

319.0

Abstract:

In this report we investigate the role of ECM proteins and growth factors in anchorage-independent survival and growth of a murine mammary carcinoma-derived cell line, designated SP1. We demonstrate that SP1 cells adhere well to FN and VN and express a number of integrins, including a5beta1, alpha v beta 3, alpha 6 beta 1, alpha 3 beta 1 and alpha 2 beta 1, the latter being upregulated under anchorage- independent conditions. We also demonstrated by partially removing FN from 7 FBS or adding FN to 1 FBS that FN is required for SP1 cell colony growth.

Descriptors:

• *GROWTH(PHYSIOLOGY)
• *BREAST CANCER
• PROTEINS
• MUTATIONS
• CLONES
• TRANSDUCERS
• CELLS(BIOLOGY)
• COLONIES(BIOLOGY)
• MAMMARY GLANDS
• TYROSINE
• PHOSPHORYLATION
• PHOSPHORUS TRANSFERASES
• METASTASIS

Subject Categories:

• Biochemistry
• Genetic Engineering and Molecular Biology
• Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE