Accession Number:

ADA330191

Title:

Mapping of a Breast Carcinoma Tumor Suppressor Gene to Chromosome 11P15.5

Descriptive Note:

Annual rept. 1 Jul 96-30 Jun 97

Corporate Author:

NORTH CAROLINA UNIV AT CHAPEL HILL

Personal Author(s):

Report Date:

1997-07-01

Pagination or Media Count:

10.0

Abstract:

Loss of heterozygosity LOH on the short arm of chromosome 11 has been demonstrated in many cancers, suggesting the presence of a tumor suppressor gene. A number of studies have narrowed the region showing LOH in breast tumors to l1p15.5. The objectives of this study are to screen breast tumor samples for LOH to further resolve the location of the putative tumor suppressor gene and to screen candidate genes from this region. We are examining LOH in our region of interest using polymorphic markers. Samples from heterozygous individuals are examined from both normal and tumor tissue. We have isolated genomic DNA from 112 tissue samples, constituting 56 matched tumor and normal pairs. These samples are being analyzed for loss of heterozygosity LOH at three separate polymorphic markers, D11S1318, D11S4088, and D11S860. The analysis of marker D11S1318 is complete and is comparable to results which have been previously been reported at 6 percent LOH. To date D11S4088 is showing 12 percent LOH and D11S860 is incomplete.

Subject Categories:

  • Genetic Engineering and Molecular Biology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE