Stromal Components of Breast Cancer Progression.
Annual rept. 15 Jun 96-14 Jun 97,
GEORGETOWN UNIV WASHINGTON DC
Pagination or Media Count:
Tumors produced in mice by native MCF-7 breast carcinoma cells or MCF-7 cells transfected with the angiogenic growth factors FOF-4, FGF-l, or VEOF were studied at early time points following tumor cell injection. Tumor cell growth or death was assessed using staining for BrdU incorporation or apoptosis under conditions of treatment with nothing, estrogen, or tamoxifen in ovariectomized mice. BrdU incorporation correlates with known hormonally-influenced growth characteristics of the tumor cells at early time points before differences in tumor size become apparent. Abundance of intratumoral blood vessels and tumor edge-associated vessels may be positively associated, and abundance of normal stromal vessels negatively associated with BrdU incorporation. Endothelial cells have been isolated from tumors produced by MCF-7 cells or the transfectants by flow cytometry utilizing monoclonal antibodies to PECAM-1. RNA produced from the isolated cells contains transcripts for PECAM-1 in greater abudance than that of EOMA cells, a murine hemangioma cell line used as a control. Transcripts for the human keratinocyte growth factor receptor expressed by the tumor cells are also present in the RNA produced from the isolated endothelial cells, indicating some degree of tumor cell contamination in the isolated cells. This RNA will be used to identify genes differentially expressed in endothelial cells participating in tumor-induced angiogenesis.
- Anatomy and Physiology
- Medicine and Medical Research