Turnover of the Stress Induced Protein, HSP7O.
Final rept. 1 Oct 94-28 Feb 97,
WYOMING UNIV LARAMIE DEPT OF MOLECULAR BIOLOGY
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The heat shock response has been studied in rainbow trout to investigate the possibility that accumulation of the heat shock protein, hsp70, may be useful as a biomarker for environmental Stress due to metal contaminated water. The heat shock response of rainbow trout has been characterized, and the rainbow trout hsp70 gene sequenced. A polyclonal antibody has been generated which is very specific for tmut hspO. Hps7O does accumulate in juvenile trout tissues including gill, liver, in response to metal Cd, Cu, Pb, Zn contaminated water and diet. Hsp7O levels in juvenile rainbow trout do not increase significantly when live rainbow trout tissues are exposed singly to environmentally relevant Cd44or Cr levels. Experiments done in Drosophila to determine the basis for the rapid turnover of hsc7O following heat shock indicate that the rate of turnover of hsc7O is determined by the temperature at which it is synthesized, suggesting the conformation of the protein is critical in the regulation of its turnover Possible differences in the folding environment which could account for this arc the temperature itself and the association of nascent hsc7O with nascent hsp7O. This turnover does not depend on the ubiquitin pathway for proteolysis.
- Stress Physiology
- Metallurgy and Metallography
- Water Pollution and Control