Loss of DNA Base Excision Repair Capacity in Initiation and Progression of Breast Cancer
Final rept. 1 Nov 94-31 Jan 97
NEW YORK UNIV MEDICAL CENTER NY
Pagination or Media Count:
Heritable alterations in Several genes including p53, ATM, BRCA1 and BRCA2 are known to play a role in cellular responses to DNA damage. Inherited or acquired alterations in these genes are thought to contribute to the development of human breast cancer. This study has focused on the purification and cloning of the mammalian homologue of the E. Coli repair enzyme endonuclease III, with the goal of determining whether this enzyme specially also plays a role in human breast cancer. Endonuclease III and its mammalian homologues are responsible for repairing several major lesions produced by oxidative damage to DNA. During the grant period, we have purified a functional analog of endonuclease III 5,000 fold from calf thymus, and cloned and expressed the human full length cDNA. Ongoing studies will allow us to determine whether this enzyme plays a specific role in genetic or environmentally produced breast cancer by characterizing its expression at the molecular level.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research