Optical Memory Potential of Photoactive Yellow Protein
Final rept. Nov 95-Jun 96
ARIZONA UNIV TUCSON OPTICAL SCIENCES CENTER
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This contract targeted improved growth of photoactive yellow protein PYP and evaluation of its optical signal processing capabilities. The growth experiments were highly successful. Ectothiorhodopsin halophila bacteria were grown and plenty of PYP isolated for the optical experiments. Then recombinant wild-type aop-protein was grown in E. coli and purified the chromophore was activated as the thiophenol ester which is very reactive with apo-protein. The reconstituted protein has properties indistinguishable from native protein. Much larger quantities of reconstituted recombinant protein can now be made in E. coli in a shorter period of time than when native protein was prepared in E. halophila. This success has made it possible to make mutants and proteins with variant chromophores, resulting in shifts in the absorption maximum and changes in the photocycle kinetics. These studies are at an early stage and should eventually lead to protein tailored to meet the requirements of photochemical device.
- Electrooptical and Optoelectronic Devices