Representational Difference Analysis of Human Breast Cancer
Annual rept. 1 Jul 96-30 Jun 97
JOHNS HOPKINS UNIV BALTIMORE MD
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The finding of homozygous deletions has been useful in localizing new tumor-suppressor genes and identifying the involvement of known suppressor genes. Four major approaches can identify homozygous deletions a candidate gene approach where losses of a particular sequence are sought, a candidate positional search where a genome scanning strategy utilizes spaced markers, a candidate locus where markers of a known homozygous deletion of one tumor system are used to specify a region for investigation in a second tumor type, and an unbiased approach such as the representational difference analysis RDA. Approaches utilizing RDA, candidate genes, and candidate loci in breast cancer are currently under investigation by our group. Using the candidate gene approach, a homozygous deletion of the DPC4 gene was identified within a breast cancer cell line. This suggested the involvement of this TGF-Beta-like pathway in breast cancer, and has stimulated the analysis of other candidate genes that may be potential members of this pathway. This approach, as well as the RDA and candidate locus methods, will contribute to our understanding of homozygous deletions and the suppressive pathways involved in this cancer.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research