Accession Number:

ADA329399

Title:

Possible Mechanisms for Regulation of Breast Tumor Micrometastasis by NME Genes

Descriptive Note:

Annual rept. 29 Apr 96-28 Apr 97

Corporate Author:

NEW YORK MEDICAL COLL VALHALLA

Personal Author(s):

Report Date:

1997-05-01

Pagination or Media Count:

24.0

Abstract:

Metastatic dissemination of primary breast tumors is controlled by positive and negative regulators. We found that expression of a catalytically inactive nucleoside diphosphate kinase B NDPK BT in MDA-MB-435 human breast carcinoma cells significantly decreases their metastatic dissemination in a nude mouse assay. Inhibition of metastatic dissemination of primary tumors formed by these cells occurs prior to formation of detectable micrometastasis. Our structural studies suggests that wild type NDPK B and highly homologous NDPK A form different oligomers that may occupy specific binding sites We hypothesize that NDPK BT displaces wild type NDPK B from specific binding sites, acting in effect as a dominant negative mutant. These results suggest that NDPK B acts as a positive regulator of metastatic potential of human tumor cells and that specific inhibition of NDPK B may be a target for potential anti-metastasis therapeutics.

Subject Categories:

  • Biochemistry
  • Genetic Engineering and Molecular Biology
  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE