Evaluation of Drug and Vaccine Candidates in the Human Malaria/Aotus Monkey Model
Annual rept. 1 Mar 96-28 Feb 97
PROMED TRADING S A MIAMI FL
Pagination or Media Count:
Chloroquine resistance CQR of the AMRU-1 Strain of Plasmodium vivax was not reversed in Aotus using prochlorperazine and chloroquine alone or in combination during a seven day regimen. An AMA-1 erythrocytic DNA vaccine, partially protected 13 monkeys, which showed a low grade parasitemia that ended in treatment due to a 40 reduction in hematocrit Hto. After rechallenge, all monkeys except for one that selfcured, had to be treated 22-29 days post inoculation PI due to their low Htos. An EBA-1 DNA erythrocytic vaccine offered partial protection in 13 monkeys that self cured 21 days PI. Multiple rechallenges with the FVO strain yielded sterile immunity. The effectiveness of the intradermal ID route of inoculation Vs the IM route at inducing high levels of antibodies in Aotus was confirmed when a distinct antigenic DNA vaccine such as HsBAg was used. The addition of Oligonucleotides to the vaccine formulation greatly increased the antibody responses observed with this antigen.
- Medicine and Medical Research