Stereochemical Determination of Selegiline Metabolites in Postmortem Biological Specimens.
FEDERAL AVIATION ADMINISTRATION OKLAHOMA CITY OK CIVIL AEROMEDICAL INST
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The Federal Aviation Administrations Toxicology and Accident Research Laboratory determines the presence of drugs, volatiles, and primary combustion gases in biological samples from aircraft accident victims and also establishes any medical condition for which the drugs might have been taken. In this study, findings related to an aircraft accident are reported. Along with biological specimens from the pilot of this fatal accident, two types of tablets found at the accident scene were submitted for analysis. These tablets were identified as levodopa and selegiline, commonly prescribed for the treatment of Parkinsons disease. Selegiline, a stereospecific compound, is biotransformed into --N-desmethylselegiline, --methamphetamine, and --amphetamine. During this process, the chiral center of the parent molecule is not affected. The latter two levorotatory metabolites cannot be easily distinguished by routine analysis from their dextrorotary isomers, which are controlled substances. Therefore, it was prudent to differentiate these isomers to prove or disprove the controlled substance categorization. Initial immunoassay drug screenings revealed the presence of amphetamine class drugs 867 ngml and amphetaminemethamphetamine 261 ngml in urine and methamphetamine 46 ngml in blood. The gas chromatography-mass spectrometry CCMS results revealed the presence of methamphetamine in the concentrations of 76 ngml of blood and 685 ogmI of urine. The level of amphetamine was 52 ngml in blood and 320 ngml in urine. To determine the stereospecificity of these amines, the isolated amines from the biosamples were derivatized by a stereospecific agent, s---N- trifluoroacetylprolyl chloride, and characterized by a CCMS method to be levorotatory. The 2.14 ratio of --methamphetamine to --amphetamine concentrations in the urine was consistent with a sele
- Military Aircraft Operations
- Medicine and Medical Research