Accession Number:

ADA326974

Title:

Electrical and Chemical Modulation of Synaptic Efficacy.

Descriptive Note:

Final rept. 1 Jul 93-30 Jun 96,

Corporate Author:

COLUMBIA UNIV NEW YORK

Personal Author(s):

• Poo, Mu-ming

Report Date:

1997-05-13

Pagination or Media Count:

5.0

Abstract:

To investigate how electric currents associated with Synaptic activity and chemical factors released by the pre- and postsynapic cells affect the efficacy of synaptic transmission, in order to understand the plasticity of synaptic functions at single neuron level. Monolayer cultures of dissociated neurons from rat hippocampi or of neurons and myocytes from Xenopus embryos are prepared, the synaptic functions are assayed by patch-clamp whole-cell recording and fluorescence imaging of intracellular calcium. Electrical activity is induced in the neuronal network by extracellular or intracellular stimuli and chemical factors are bath- or focally-applied to the synapses.

Descriptors:

• *CHEMICAL PROPERTIES
• *ELECTRICAL PROPERTIES
• *ELECTRIC CURRENT
• *SYNAPSE
• FLUORESCENCE
• RATS
• NETWORKS
• CHEMICALS
• PLASTIC PROPERTIES
• NERVE CELLS
• STIMULI
• MODULATION
• EMBRYOS
• CELLS(BIOLOGY)

Subject Categories:

• Anatomy and Physiology
• Inorganic Chemistry
• Electrical and Electronic Equipment

Distribution Statement:

APPROVED FOR PUBLIC RELEASE